Direct visualization of red fluorescent lipoproteins indicates conservation of the membrane sorting rules in the family Enterobacteriaceae.

Fluorescent Contrast agent Based on Graphene Quantum Dots Decorated Mesoporous Silica Nanoparticles for Detecting and Sorting Cancer Cells

Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins. Material and Methods: We adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 c...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Construction of Various γ34.5 Deleted Fluorescent-Expressing Oncolytic herpes Simplex type 1 (oHSV) for Generation and Isolation of HSV-Based Vectors

Background: Oncolytic herpes simplex virus (oHSV)-based vectors lacking γ34.5 gene, are considered as ideal templates to construct efficient vectors for (targeted) cancer gene therapy. Herein, we reported the construction of three single/dually-flourescence labeled and γ34.5-deleted, recombinant HSV-1 vectors for rapid generation and easy selection/isolation of different HSV-Based v...

Red cell membrane protein abnormalities as defined by sds-page among patients with anaemia in a west african region hospital practice

Background: Erythrocytes require an ability to deform and to withstand shear stress while negotiating the microcirculation. These properties are largely due to their excess surface area per volume and the characteristics of the membrane’s protein. Deficiencies of these proteins are associated with chronic haemolysis. Methods: This was a cross sectional  study aimed at determining the prevalenc...

Preparation and Characterization of Thermoresponsive In-situ Forming Poloxamer Hydrogel for Controlled Release of Nile red-loaded Solid Lipid Nanoparticles

Preparation and characterization of thermoresponsive in-situ forming poloxamer hydrogel for controlled release of Nile red-loaded solid lipid nanoparticles. Nanoparticles (NPs) are cleared rapidly from systemic circulation and do not provide sustained action in most cases. To solve this problem, this investigation introduces an erodible in-situ forming gel system as potential vehicles for prolo...